Blood mononuclear cells (HNC) are commonly isolated by Ficoll-Hypaque density centrifugation (FH) prior to monoclonal antibody staining and flow cytometric lymphocyte phenotype analysis. We have found ...

Accurate and timely diagnosis of inborn errors of immunity (IEI) is essential for improving patient outcomes. Flow cytometry is widely recommended for robust and sensitive IEI screening; however, no ...

Before setting a gating strategy, it is vital to know the properties of cells to be analyzed, such as cell size, the relative expression of markers and whether artifacts are likely to have been ...

A method for high-resolution analysis of the human karyotype by flow cytometry has been developed. Metaphase chromosomes are prepared from short-term peripheral blood cultures, stained with ethidium ...

Flow cytometry is a way to look closely at the features of cells or particles. A sample of blood or tissue goes into a machine called a cytometer. In less than a minute, a computer can analyze ...

Around the same time, Mack Fulwyler, an engineer working at Los Alamos National Laboratory, needed to separate particles, so he drew on existing techniques to create droplets to separate cells from a ...

One of the primary objectives for the application of flow cytometry in any testing environment should be measurement assurance, i.e., the generation of reliable and reproducible results. This goal can ...

Flow cytometry can be used to measure a number of characteristics of a population of cells and refers to the process of passing cells individually through a single or series of laser beams adjacent to ...